Structure of a homofructosan from Saussurea costus and anti-complementary activity of its sulfated derivatives
Author(s)
Author(s)
Fan, H.
Liu, F.
Shi, S.
Wang, S.
Date Issued
2014
Publisher
Elsevier
Journal
Carbohydrate Polymers
Volume
105
Start page
152
End page
160
Abstract
A homogeneous water-soluble polysaccharide APS-W1, (2 → 1)-β-d-fructofuranosan, with an average molecular weight of 3.9 kDa, was isolated and characterized from the roots of Saussurea costus. Five sulfated derivatives of APS-W1 with different degrees of sulfation were prepared and they showed strong inhibitory effect on the complement activation through the classical pathway (CP50: 2.2–18.9 μg/mL; 8.3 μg/mL for heparin) and alternative pathway (AP50: 11.4–115.8 μg/mL; 89.2 μg/mL for heparin). Mechanism studies by using complement-depleted sera indicated that sulfated derivatives with different positions of sulfation targeted to different complement proteins. Meanwhile the sulfated derivatives have limited anticoagulant effect based on re-calcification time and thrombin time. These results suggested that the sulfated derivatives prepared from APS-W1 could be promising potential complement inhibitors for the treatment of diseases caused by an over-activated complement system.
SFU Affiliated Publication
No
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