Bligh, Annie Sim WanAnnie Sim WanBlighWu, T.Gu, L.-H.Wang, Z.-T.Liu, H.-P.Cheng, X.-M.Branford-White, C. J.Hu, Z.-B.2021-12-092021-12-092005https://repository.sfu.edu.hk/handle/sfu/2023A HPLC-UV method for the quantification of six major isoflavonoids, calycosin 7-O-β-d-glucoside (<b>1</b>), formononetin 7-O-β-d-glucoside (<b>2</b>), (6αR, 11αR) 3-hydroxy-9,10-dimethoxypterocarpan-3-O-β-d-glucoside (<b>3</b>), 7,2′-dihydroxy-3′,4′-dimethoxyisoflavan-7-O-β-d-glucoside (<b>4</b>), calycosin (<b>5</b>) and formononetin (<b>6</b>), in Radix Astragali (<i>Huangqi</i>) was developed and validated. The method was proven to be sensitive, specific, accurate and precise, as well as effective and easy.enjournal article10.1016/j.fitote.2004.11.006