Bligh, Annie Sim WanAnnie Sim WanBlighWang, S. C.Zhu, C. L.Shi, S. S.Wang, Z. T.Hu, Z. B.Crowder, J.Branford-White, C.Vella, C.2021-12-092021-12-092008https://repository.sfu.edu.hk/handle/sfu/2008China is a major producer of oats; the annual harvested area of 350,000 ha yields approximately 465,000 tons, giving an average yield of 1.33 tons/ha. The bran is not used for animal feed as it is of poor digestibility and low nutritive content and is considered a waste byproduct. Therefore, it is advantageous to produce a value-added product from the bran. We extracted the native polysaccharide, a linear (1–3)-, (1–4)-linked β-glucan (OBG) from the oat bran and synthesized a sulfated derivative OBGS containing 36.5% sulfate. OBGS had potent activity against a primary isolate of human immunodeficiency virus (HIV-1) in peripheral blood mononuclear cells at a concentration (EC50 = 5.98 ×10⁻⁴ µM) approximately 15,000 times below its cytotoxic concentration. OBGS was also active postinfection (EC₅₀ = 5.3 ×10⁻⁴ µM) and protected pretreated peripheral mononuclear cells (EC₅₀ = 5.2 ×10⁻² µM) washed free of the compounds prior to infection. Thus, OBGS has potential as a vaginal microbicide and is the first such report for oat bran derived sulfated̃ β-glucan.enjournal article10.1021/jf072888h