Flavonoids by HPLC
Author(s)
Author(s)
Ogegbo, O.
Wang, Z.-T.
Date Issued
2013
Publisher
Springer
Related Publication(s)
Natural products: Phytochemistry, botany and metabolism of alkaloids, phenolics and terpenes
Start page
2107
End page
2144
Abstract
Flavonoids are secondary plant metabolites that are synthesized via the shikimate pathway. HPLC has been an important tool for the separation of these metabolites in the last 4 decades. The coupling of HPLC with a number of detection technologies either online, in tandem, or off-line enables the identification of flavonoids in plant, food, and biological samples. This chapter provides an overview of flavonoid analysis by HPLC, including extraction of flavonoids in both aglycones and glycosides, separation by a selection of stationary and mobile phases, and finally, detection and identification by UV–VIS, fluorescence, electrochemical, mass spectrometry, and NMR spectroscopy.
SFU Affiliated Publication
No