Simultaneous determination of amino acids in discrete brain areas in Suncus murinus by high performance liquid chromatography with electrochemical detection
Author(s)
Author(s)
Lin, G.
Yamamoto, K.
Yew, D. T. W.
Rudd, J. A.
Date Issued
2010
Publisher
Elsevier
Journal
Journal of Pharmaceutical and Biomedical Analysis
Volume
53
Issue
3
Start page
705
End page
709
Abstract
An improved and simple reversed-phase high performance liquid chromatography method with electrochemical detection for the simultaneous determination of amino acids in brain tissue of Suncus murinus was developed. Homogenates from 5 different brain areas were derivatized with o-phthalaldehyde in the presence of sodium sulphite. Subsequent separation was achieved using linear gradient elution over 30 min. The derivatives were stable for up to 20 h at 4 °C. The method was accurate, reproducible, and showed good linearity. The recoveries were >88% for aspartate, glutamine, glutamate, glycine and γ-aminobutyric acid, with the limit of quantification varying from 5 to 30 pmol. The method was successfully applied for the measurement of amino acids under fed and fasted conditions.
SFU Affiliated Publication
No
Availability at SFU Library
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